Fluorescence and Structured Illumination Microscopy
Webinar Series for Africa
We are pleased to invite you to a series of webinars which have been put together especially for you, our customers on the African continent. During the series, we will focus on the overarching topic of fluorescence and structured illumination microscopy including the latest advances in the light microscopy ecosystem covering state-of-the-art technologies, efficient workflows, and sample preparation methods.
Please see below all topics, dates and times and register for as many webinars as you would like to attend.
Program Overview
| Webinar Topic | Speaker | Date | |
|---|---|---|---|
Introduction to Fluorescence Microscopy and Sample Preparation |
Mrs. Soné Hendriks |
May, 3rd |
|
Imaging of Living Samples - New Technologies for Fast and Gentle Image Acquisition |
Mrs. Soné Hendriks |
May, 10th |
|
Countless Possibilities of Confocal Microscopy |
Dr. Chris Power |
May, 17th |
|
Discover a New Lightsheet Microscope for Multiview Imaging of Living and Cleared Specimens |
Dr. Courtney Akitake |
May, 24th |
1.00 - 2.00 p.m. (CAT)
Introduction to Fluorescence Microscopy and Sample Preparation
Appropriate sample preparation is necessary to ensure high quality images are captured. Since the fluorescent probes have a major impact on microscopy, learn about how fluorescent dyes and proteins are important and see how they can be used in functional and structural studies.
- Focus on the basics of fluorescence microscopy
- Discuss the multiple steps of sample preparation for fluorescence microscopy
- Discuss the workflow, fluorescence labelling and staining as well as tips for choosing the best fluorophore
1.00 - 2.00 p.m. (CAT)
Imaging of Living Samples - New Technologies for Fast and Gentle Image Acquisition
Being able to image living samples has allowed researchers to observe complex morphogenic processes such as the development of an organism over time, but it comes with its own challenges. Living samples are often delicate and finding the optimal imaging set up is critical for the success of the experiment. In this webinar we will discuss the imaging parameters that are important for your live cell imaging experiments and deduce the best imaging technique from them.
- Focus on a variety of different examples and evaluate which method would be the most suitable, leading to the best results
- Review imaging of samples from single cells in culture, all the way to imaging of larger 3D model organisms, such as zebrafish and cricket embryos
- Discuss Widefield Microscopy with Deconvolution, Confocal Microscopy and Airyscan 2 Multiplex, Multiphoton microscopy, Structure Illumination Microscopy, and Lightsheet Microscopy
Webinar 3 - Tuesday, 17th May 2022
1.00 - 2.00 p.m. (CAT)
Countless Possibilities of Confocal Microscopy
This webinar discusses the basic principles of confocal microscopy and history. The large variety of applications addressed by Laser Scanning Microscopes is addressed including multicolour imaging, FRAP, FCS, FLIM, etc. Software options for reproducible and automated data acquisition and processing are also discussed.
- Focus on what factors contribute to sensitive quantitative confocal microscopy
- Discover experimental capabilities beyond pure imaging
- See how software can automate data acquisition and analysis
Webinar 4 - Tuesday, 24th May 2022
1.00 - 2.00 p.m. (CAT)
Discover a New Lightsheet Microscope for Multiview Imaging of Living and Cleared Specimens
Light Sheet Fluorescence Microscopy (LSFM) is a powerful alternative to traditional epi-illumination techniques, especially for 3D imaging within whole live organisms and large tissue explants. By selectively illuminating the observed optical section with a thin sheet of light, photo-damage and bleaching are reduced to a minimum, making light sheet microscopy ideal for nondestructive imaging of fragile samples. Due to the speed and efficiency of image acquisition, LSFM is particularly well suited for volume imaging of samples that have been rendered transparent through various tissue-clearing methods.
- Receive a brief introduction to light sheet microscopy and a short overview of optical clearing techniques
- Discover a demonstration of how common challenges of cleared tissue imaging can be solved with features such as flexible sample mounting and the ability to accommodate different optical clearing agents
- Explore several tools for micro- to meso-scale imaging, including a variety of long working distance objectives, that make light sheet microscopy an extremely versatile technique for imaging cleared tissues